Western Blot:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Nucleic Acid Electrophoresis:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Molecular Weight:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Staining:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Activation Assay:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Incubation:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Concentration Assay:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Recombinant:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Flow Cytometry:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Control:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Two Tailed Test:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Expressing:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Membrane:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Fluorescence:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Quantitative Proteomics:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Transfection:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Knockdown:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
CRISPR:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Over Expression:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Quantitative RT-PCR:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Inhibition:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
CCK-8 Assay:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
Migration:Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis
Article Snippet: The human GBM cell line M059K (American Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco).Type Culture Collection (ATCC), Manassas, VA, USA, Cat#: CRL-2365, RRID: CVCL_0401) was maintained in DMEM-F12 (Cleveland Clinic Cell Services Media Core, Cleveland, OH, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 1% antibiotic–antimycotic (Gibco), and 1% minimal essential media non-essential amino acids (Gibco). ... The human GBM cell lines A172 (ATCC, Cat#: CRL-1620, RRID: CVCL_0131) and T98G (ATCC, Cat#: CRL-1690, RRID: CVCL_0556) were maintained in high glucose DMEM (Cleveland Clinic) containing 10% FBS and 1% antibiotic–antimycotic.. Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).Two different primary non-tumorigenic human astrocyte cell isolates, denoted as Astro-1 (Cell Systems Corporation, Kirkland, WA, USA, Cat#: ACBRI-371) and Astro-2 (Celprogen, Torrance, CA, USA, SKU: 36058-01), were maintained in high glucose DMEM supplemented with 10% FBS, 1% antibiotic–antimycotic, and 0.2% Bac-off (Cell Systems).
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27.
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27
Article Snippet: Human GBM cell lines A172 (RRID: CVCL-0131), U87MG (HTB-14), an unidentified GBM (RRID: CVCL_0022), CRL2611, HTB-12, HTB-138, HTB-15, and HTB-16 were acquired from the American Type Culture Collection (ATCC).. The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.The T98G and LN18 cell lines were generously supplied by Dr. Erwin Van Meir of the University of Alabama at Birmingham.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1.
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO2/95% air atmosphere.
Article Title: LncRNA H19 acts as a ceRNA to promote glioblastoma malignancy by sponging miR-19b-3p and upregulating SERPINE1
Article Snippet: The human GBM cell lines A172 and U87MG were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.A172 cells were cultured in RPMI-1640 (Hyclone, Marlborough, MA, USA) supplemented with 10% FBS (Hyclone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator with a 5% CO 2 /95% air atmosphere.
Article Title: Circadian rhythm related genes signature in glioma for drug resistance prediction: a comprehensive analysis integrating transcriptomics and machine learning
Article Snippet: The normal human brain glial cell line HEB and the human GBM cell lines A172, SHG44, U251, and H4 were procured from the American Type Culture Collection (ATCC; Manassas, USA).. These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.These cells were cultured in RPMI1640 medium, supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin.
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